Suggested Sampling Instructions for Non-Food Contact Surfaces
Sample Shipping Instructions
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Personnel should thoroughly wash hands with soap and water before and after sample collection.
Materials:
- Whirl-Pak Bag, Sterile Sponge (pre-wetted), Sterile Gloves (Pre-packaged) 1 per sampling site
- Predetermined sampling site of approximately 10″ x 10″ (100 in2), for more intricate surface areas, an equivalent area estimation or a “per part” sample is acceptable.
- Permanent Ink Marker
Procedure:
- Separate the glove/sponge portion from the sponge Whirl-Pak at the perforation.
- Tear off the clear, perforated strip at the top of the Whirl-Pak bag.
- Put on the sterile glove.
- a. Remove the sterile glove from the pouch by the top edge without contaminating (touching, breathing on, contacting, etc.) the glove.
- b. Remove a glove by holding it from the wrist-side opening. Avoid any contact with the outer surface of the glove. Insert clean hand into glove, taking care not to puncture the glove.
- Open the bag containing the sponge, wearing sterile gloves, being careful not to touch sponge to anything but the gloved hand.
- With gloved hand, remove sponge from the bag.
- Using firm, even pressure, move sponge slowly and thoroughly over sampling area, first horizontally, then vertically.
- Return sponge back to Whirl-Pak bag, taking care not to contaminate the sponge or the bag with the ungloved hand.
- Close the bag by folding the top down three times and bending the wire ends over onto the bag.
- Label the sample using a permanent ink marker.
- Keep sample(s) at a refrigerated temperature (35°-40°F) until submitted to the laboratory for analysis.
Suggested Sampling Instructions for Food Contact Surfaces
Personnel should wash hands thoroughly with soap and water before and after sample collection.
Materials:
- Environmental swab with Neutralizing Solution (1 per sampling site)
- Permanent Ink Marker
- Predetermined sampling site of approximately 4″ x 4″ (~100 cm2), or for more intricate surface areas, an equivalent area estimation or a “per part” sample is acceptable.
- Gloves (non-sterile)
Procedure:
- With gloves on, remove swab from sterile packaging.
- Carefully unscrew cap of sampling device – swab is attached to the lid of the cap.
- Gently press out excess solution from sampling swab by pressing the swab against the inside wall of the tube with a rolling motion.
- Hold swab at an approximate 30° angle from the sampling surface, taking care not to contaminate any part of the swab or the sampling site.
- Using firm, even pressure, move the swab slowly and thoroughly over the entire sampling area, rewetting the swab tip with the Neutralizing Solution as needed. First horizontally, then vertically.
- After sampling is complete, carefully put swab back into vial and close cap tight.
- Label the sample using a permanent ink marker.
- Keep sample(s) at a refrigerated temperature (35°–40°F) until submitted to the laboratory for analysis.
Sampling supplies available! Please contact the laboratory prior to sample submission to check availability for non-standard and/or expedited test requests.
Chain of Custody Documentation
Food Microbiology Labs
- Houston, TX
- Indianapolis, IN
- St. Louis, MO
- Tampa, FL
- Orlando, FL
- Atlanta, GA
- Cinnaminson, NJ
- Raleigh, NC
